Laboratory of Biotechnology and Biomaterials Research

We deal with biotechnological production, analysis, characterization and application of various biomolecules and biomaterials and, furthermore, we are working on development of delivery systems for selected biologically active substances. The research group consists of students of all university levels (undergraduates and PhD. students) as well as employees of the faculty. Therefore, the less skilled students can gain a lot of support and experience from the cooperation within the group.

Presentation of laboratory.

Contact:  Assoc. Prof. Ivana Márová


RESEARCH PROgram

Advanced Organic Materials and Biomaterials


RESEARCH Focus

Microbes are not only tiny living organisms but they also possess many fascinating capabilities. Some of them are more or less danger or unpleasant such as their ability to cause diseases, however, there is also great value provided by microorganism. They can produce various substances, which may not be reasonably produced in other ways, or they are able to degrade many substances, which are extremely resistant to other environmental factors. This bright side of microorganisms is in the middle of our attention. We are focused on microbial production of various metabolites which might have commercial potential such as enzymes, antioxidants, vitamers (carotenoids, ergosterol, coenzyme Q) and other metabolites that can be produced from inexpensive sources by special yeast strains. Further, we aim at production of polyhydroxyalkanoates. These unique bioplastics are produced by a wide variety of bacterial strains. Their mechanical properties are similar to these of traditional plastics of petrochemical origin, nevertheless, unlike them polyhydroxyalkanoates are easily biodegradable. Thus, their disposal does not represent environmental problem. In both research streams, we are interested in involvement of carotenoids and polyhydroxyalkanoates into the stress response of microorganisms and, oppositely, we study physiological stress as a tool for improvement of the process of the biotechnological production of these compounds. Furthermore, we are working on development of polyhydroxyalkanoates and other biomaterials-based delivery systems for selected biologically active substances. Last but not least, long-lasting research is aimed at analysis of foods and beverages (such as wine or beer), which are traditionally produced by using microorganisms. These analyses include tests of genotoxicity/antimutagenity of matrices by special yeast strain of S. cerevisiae.


FACILITIES AND EXPERTISE

The laboratory is equipped for cultivation of the wide variety of biotechnologically utilizable microorganisms and for analysis of broad spectrum of biomolecules. A high number of analyses is performed by HPLC including HPLC/PDA/ESI-MS. Further, we possess equipment for isolation, purification and molecular characterization of DNA and proteins.